mouse monoclonal antibody against pgp9 5  (Servicebio Inc)

Journal: Open Medicine

Article Title: Proteoglycan-depleted regions of annular injury promote nerve ingrowth in a rabbit disc degeneration model

doi: 10.1515/med-2021-0363

Figure Lengend Snippet: PGP9.5 immunohistochemical staining at 6 months after surgery. In the intact AF, PGP9.5-positive nerves were distributed sparsely in the lamellae and adjacent connective tissue of the outer AF (a). Small nerves in the vicinity of the outer AF were distributed along the fissures but rarely invaded the deeper inner AF in the AF defect group (b). PGP9.5-positive nerve fibers were present within the newly generated tissue of inner AF (c). In the PLGA/fibrin/chABC discs, more sprouting nerve fibers were predominantly identified in the vicinity of vascularized repair tissue and extended further into the inner AF (d). White arrows indicate areas of PGP9.5-positive nerve fibers. Long white arrows indicate the radial direction (from nucleus to outer annulus). R, radial.

Article Snippet: The sections were then blocked with hydrogen peroxide and 25% of normal bovine serum albumin (BSA)/tris buffered saline (TBS) and incubated overnight at 4°C with the primary mouse monoclonal antibody against human PGP9.5 (diluted 1:80, Abcam, Cambridge, GB).

Techniques: Immunohistochemical staining, Staining, Generated